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Recent
times have seen a phenomenal rise in the numbers of snoRNAs without appreciable
increase in the corresponding counts of their conventional targets. One such
snoRNA, HBII-52, bears perfect 18nt complementarity to an unconventional
Serotonin receptor 2C pre-mRNA target in its alternatively spliced exon Vb. We
have earlier shown that HBII-52 regulates the receptor’s splicing by masking a
silencing element. Lack of HBII-52 expression in people with Prader Willi
Syndrome generates less potent receptor which couples inefficiently to the
second messengers. The misregulated receptor signaling contributes to the
development of the Syndrome. Antisense elements of 14 out of the 47 copies of
HBII-52 found in the human genome deviate 1-3 nucleotides from the consensus
sequence. Toleration of a few mismatches in the antisense element could identify
through computational searches several other pre-mRNAs as the putative target.
We work towards understanding the functions of snoRNAs expressed from the
maternally imprinted SNURF-SNRPN locus. We investigate the possibility that
snoRNAs in general could act as versatile modulators of alternative splicing and
in turn regulate gene expression.
Academic
Training
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1997-2002 |
Bachelor’s and Master’s (Dual degree) in
Biochemical Engineering and Biotechnology, Indian Institute of
Technology, Delhi. India. |
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2001-2002 |
Research for Master’s dissertation in
Malaria Group, International Center for Genetic Engineering and
Biotechnology (ICGEB), New Delhi, India. |
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Jun’ 2002-Mar’ 2006 |
Graduate student at Stamm’s lab, University
of Erlangen-Nürnberg, Germany. |
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Mar’2006- |
Postdoctoral position at Stamms’s lab. |
Publications
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Kishore, S. and Stamm,
S. The snoRNA HBII-52 regulates alternative splicing of the serotonin
receptor 2C. Science, 2006 Jan 13;311(5758):230-2. |
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Tang Y., Novoyatleva T., Benderska N., Kishore S., Thanaraj A.
and Stamm S. (2005)
Analysis of Alternative Splicing
In Vivo using Minigenes.
Handbook of RNA
Biochemistry, WILEY-VCH Verlag
GmbH & Co. KHaA, Weinheim. |
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Bergin-AM, Balder B,
Kishore S, Swärd K, Hahn-Zoric M, Löwhagen O, Hanson LA, Padyukov L.
Common variations in the IL4R gene affect splicing and influence natural
expression of the soluble isoform. Human Mutations, (in
press). |
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Mohmmed A, Kishore S, Patra KP, Dasaradhi PV, Malhotra P, Chauhan
VS. Identification of karyopherin beta as an immunogenic antigen of the
malaria parasite using immune mice and human sera. Parasite Immunol.
2005 May; 27(5):197-203. |
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Mohmmed A, Kishore S,
Dasaradhi PV, Patra K, Malhotra P, Chauhan VS. Cloning and
characterization of Plasmodium falciparum homologs of nuclear import
factors, karyopherin alpha and karyopherin beta. Mol Biochem
Parasitol. 2003 Apr 3; 127(2):199-203. |
Tel: +49 9131 8524617
Fax: +49 9131 8524605
eMail
Shivendra Kishore
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